Zhou Xu, Handong Wang, Bulei Wang, Liang Fu, Ming Yuan, Jing Liu, Lijun Zhou, Chunbang Ding*.Characterization and antioxidant activities of polysaccharides from the leaves of Lilium lancifolium Thunb[J]. International Journal of Biological Macromolecules, 2016, 92:148–155.
Abstract
In this study, LLP-1, LLP-2, and LLP-3 three novel polysaccharide fractions were purified from theleaves of Lilium lancifolium, and their physicochemical characterizations and antioxidant properties wereinvestigated by chemical methods, high performance gel permeation chromatography (HPGPC), highperformance liquid chromatography (HPLC), fourier transform infrared spectrometry (FT-IR), scanningelectron microscopy (SEM), and DPPH radical assay, hydroxyl radical assay, superoxide radical assayand ferrous ion chelating assay, respectively. Results showed that LLP-1, LLP-2, and LLP-3 had low pro-tein and uronic acid contents, meanwhile, their weight-average molecular weight were estimated tobe 2.25 × 106, 2.02 × 106, and 2.08 × 106Da, respectively. Mannose, rhamnose, glucuronic acid, galac-turonic acid, glucose, galactose, arabinose glucose and galactose were the major monosaccaheridescomponents presenting in the polysaccharide fractions. Three polysaccharide fractions were not observedtriple-helical conformation, while possessed variant surface structure. In addition, three polysaccharidefractions all exhibited significantly scavenging activities against free radical and chelate Fe2+ in vitro.
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